This issue examines how architecture, urbanism and the natural world can be aligned beyond the established frameworks of industrialization to catalyse regenerative practices that can restore the health of our living spaces and habitats. These next-generation sustainable approaches spatalise fundamental relationships between ecosystems, technologies, and culture in ways that facilitate the cyclical flow of matter within the biosphere. Changing the impacts of human development in ways that support a culture of life, architecture and the city become sites for developing mutually supportive relations between all life in the context of a changing world and the profound damage wrought by the Anthropocene. Proposals in this issue seek a diverse, experimental and varied approach towards regenerating the health of our planet’s ecosystems by designing new encounters, methods, tools, artefacts, narratives and systems across micro-, human and macroscale, which offer new insights and regenerative strategies that facilitate symbiotic relations across the living world.